Comparison of SARS-cov-2 RdRp protein with SARS-cov RdRp protein

The World Health Organization (WHO) declared, on January 30, 2020, a public health emergency of international scope because of the emergence of a new virus called SARS-cov-2. This new virus belongs to the coronavirus family and has a protein called RNA-dependent RNA polymerase (RdRp) which is responsible for the replication of viral RNA. RdRp protein is one of the most primary targets for antiviral drug discoveries. The aim of this paper was to compare the amino acid sequence of the RdRp of SARS-cov-2 with that of SARS-cov. Thus, we found that there is a 96% sequence similarity between them. Indeed, there is only a difference at the level of 32 amino acids. Interestingly, only one residue at C Motif and two residues at D Motif are different. However, all the residues of the motifs A, B, E, F and G are 100% identical with those of SARS-cov-RdRp

Cytogenetic analysis and SRY gene detection in four moroccan brothers with Disorder of sex development

The objective of this study is to determine the genetic cause of the disorder of sex development on Moroccan family. Indeed, the genomics and human genetics laboratory at the Pasteur Institute of Morocco recruited four brothers with ambiguous genitalia. Cytogenetic analysis of the first brother revealed the presence of a chimeric karyotype 46, XX/46, XY with the presence of the SRY gene. The other three brothers present a normal female karyotype 46, XX with the presence of the SRY gene

How is the SARS-cov-2 virus transmitted ?

Since December 2019, the recent outbreak of coronavirus disease (COVID-19) has continued to spread drastically around the world. To date, no approved drug or vaccine is available to treat or prevent this new coronavirus (SARS-CoV-2) infection.Unprecedented global effort has been made by researchers to understand the various routes of SARS-CoV-2 virus transmission in order to effectively preventthe contamination. In this review, we discuss the updated literature regarding the different modes of SARS-CoV-2 transmission

Hybrid approach of the fuzzy C-Means and the K-Nearest neighbors methods during the retrieve phase of dynamic case based reasoning for personalized Follow-up of learners in real time

The goal of adaptive learning systems is to help the learner achieve their goals and guide their learning. These systems make it possible to adapt the presentation of learning resources according to learners' needs, characteristics and learning styles, by offering them personalized courses. We propose an approach to an adaptive learning system that takes into account the initial learning profile based on Felder Silverman's learning style model in order to propose an initial learning path and the dynamic change of his behavior during the learning process using the Incremental Dynamic Case Based Reasoning approach to monitor and control its behavior in real time, based on the successful experiences of other learners, to personalize the learning. These learner experiences are grouped into homogeneous classes at the behavioral level, using the Fuzzy C-Means unsupervised machine learning method to facilitate the search for learners with similar behaviors using the supervised machine learning method K- Nearest Neighbors

Our System IDCBR-MAS: from the Modelisation by AUML to the Implementation under JADE Platform

This paper presents our work in the field of Intelligent Tutoring System (ITS), in fact there is still the problem of knowing how to ensure an individualized and continuous learners follow-up during learning process, indeed among the numerous methods proposed, very few systems concentrate on a real time learners follow-up. Our work in this field develops the design and implementation of a Multi-Agents System Based on Dynamic Case Based Reasoning which can initiate learning and provide an individualized follow-up of learner. This approach involves 1) the use of Dynamic Case Based Reasoning to retrieve the past experiences that are similar to the learner’s traces (traces in progress), and 2) the use of Multi-Agents System. Our Work focuses on the use of the learner traces. When interacting with the platform, every learner leaves his/her traces on the machine. The traces are stored in database, this operation enriches collective past experiences. The traces left by the learner during the learning session evolve dynamically over time; the case-based reasoning must take into account this evolution in an incremental way. In other words, we do not consider each evolution of the traces as a new target, so the use of classical cycle Case Based reasoning in this case is insufficient and inadequate. In order to solve this problem, we propose a dynamic retrieving method based on a complementary similarity measure, named Inverse Longest Common Sub-Sequence (ILCSS). Through monitoring, comparing and analyzing these traces, the system keeps a constant intelligent watch on the platform, and therefore it detects the difficulties hindering progress, and it avoids possible dropping out. The system can support any learning subject. To help and guide the learner, the system is equipped with combined virtual and human tutors

Effect of geographic origin in mineral composition of zeen oak acorns (Quercus canariensis Willd)

The aim of the current study was to investigate the effect of geographic origin in mineral compositions on the Q. canariensis acorns. The results showed that there were significant (p < 0.5) differences between populations for macronutrients Sodium (Na), Calcium (Ca), Potassium (K)) except for Phosphorus (P) and for all studied micronutrients: Iron (Fe), Manganese (Mn) and zinc (Zn). Potassuim contents of acorn ranged from 5,638 to 7,667 mg/g MS with highest being for Nefza acorn and lowest for Bni Mtir ones. It is the highest macronutrients for all population. However Iron was the most abandon micronutrient, varied widely from 0,491 mg/g MS (Bni Mtir) to 1,230 mg/g MS (Nefza). No significant differences were recorded for Heavy metal. The concentrations of Chromium (Cr), Nickel (Ni) and Cobalt (Co) in acorns flour are null. On the other hand Cadmium (Cd) and Copper (Cu) were found but with concentrations which were much lower than that the acceptable limits for herbs and spices by WHO

Y chromosome microdeletions in infertile Moroccan males: 10 years laboratory experience in AZF deletions

Genetic causes of male infertility are abnormalities in chromosome numbers and/or structures, Y-chromosome deletions and gene mutations. Genetic screening of male infertility is rarely done in our country. The purpose of the study was to investigate the frequencies and types of Y chromosome microdeletions in infertile men, based on studies done in the Human Genetics Laboratory of the Pasteur Institute in Morocco.A total of 543 infertile men were screened for Y chromosome microdeletions.The prevalence of AZF Y-chromosome microdeletions among infertile men range from 3% to 10% depending on patients selected. The most frequent microdeletions were detected in the AZFc region, followed by AZFbc, AZFb, AZFa, AZFab.These results indicate the need for Y chromosome microdeletion screening for better management of infertile patients.We hope to encourage use of genetic diagnosis and also research in this field to initiate collaboration for clinical management and appropriate genetic diagnosis and counselling for male infertility

Dominant ACO2 mutations are a frequent cause of isolated optic atrophy.

Biallelic mutations in ACO2, encoding the mitochondrial aconitase 2, have been identified in individuals with neurodegenerative syndromes, including infantile cerebellar retinal degeneration and recessive optic neuropathies (locus OPA9). By screening European cohorts of individuals with genetically unsolved inherited optic neuropathies, we identified 61 cases harbouring variants in ACO2, among whom 50 carried dominant mutations, emphasizing for the first time the important contribution of ACO2 monoallelic pathogenic variants to dominant optic atrophy. Analysis of the ophthalmological and clinical data revealed that recessive cases are affected more severely than dominant cases, while not significantly earlier. In addition, 27% of the recessive cases and 11% of the dominant cases manifested with extraocular features in addition to optic atrophy. In silico analyses of ACO2 variants predicted their deleterious impacts on ACO2 biophysical properties. Skin derived fibroblasts from patients harbouring dominant and recessive ACO2 mutations revealed a reduction of ACO2 abundance and enzymatic activity, and the impairment of the mitochondrial respiration using citrate and pyruvate as substrates, while the addition of other Krebs cycle intermediates restored a normal respiration, suggesting a possible short-cut adaptation of the tricarboxylic citric acid cycle. Analysis of the mitochondrial genome abundance disclosed a significant reduction of the mitochondrial DNA amount in all ACO2 fibroblasts. Overall, our data position ACO2 as the third most frequently mutated gene in autosomal inherited optic neuropathies, after OPA1 and WFS1, and emphasize the crucial involvement of the first steps of the Krebs cycle in the maintenance and survival of retinal ganglion cells